transgene was conducted on DNA extracted from 1 mm pieces of putative transgenic plantlets using the PlatinumDirect PCR Universal Master Mix . Primers NPT-F and NPT-R were used to amplify a 691 bp segment of. Isogenic wild-type plants were used as negative controls and pHA194 was used as a positive control.
Edited TG-20e arose from transformation event TG-20 that had been transformed/edited via biolistics. Images were acquired on Nikon Instruments point scanning Ti2-A1R confocal microscope using 40 × 1.25NA water immersion objective. Two GaAsP detectors were used to capture the green and red signals. Corresponding leaf section and plant images were captured using a smartphone camera.
UofIllinois Just cause we can, doesn't mean we should.
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